1.
Epidemiological Patterns Of Brucellosis In Sheep, Goats And Human Beings
by Jafar Pasand Masoumi | Sh. Muhammad Amin | Dr. Rashid | Mr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1986Dissertation note: The epidemiological patterns of brucellosis were studied on 1554 serum samples (goats, 500, sheep 532, human being 522) and 1027 milk samples (goats 527, sheep 500) for utilizing this information in effectively controlling or eradicating this disease from livestock and human population in Pakistan.
Various tests used comprised slide test, standard tube agglutination test and milk ring test. The antigen for serum agglutination test and slide test was the Standardised Brucella abortus antigen of Veterinary Research Institute, Lahore. For milk ring test haematoxylin stained Brucella abortus antigen developed at Veterinary Research Institute, Lahore was used. An overall incidence of 3.00%, 1.69% and 0.95% was recorded respectively in goats, sheep and human beings by serum agglutination test. The milk ring test gave an infection rate of 9.10% in goats and 7.6% in sheep. The influence of various epidemiolo- gical factors was confirmed and in goats a higher incidence by serum agglutination test was detected in females (3.65%), Teddy breed (.3.93%) and in animals of 4 years and above age group (9.52%). In sheep a higher incidence was recorded in females (3.10%), non-descript breed (3.18%) and in animals of 4 years and above age group (2%). In human beings a higher incidence was observed in males (1.27%), 20-29 years age group (1.86%) butchers (8.33%) and persons in habit of consuming raw milk
(5.40%).
The findings of the present study have revealed an alarming proportion of the disease incidence in our sheep and goats and confirmed the presence of infection in human being. Thjs calls for an immediate response of the experts, and introduction of appropriate brucellosis control measures both in livestock and human population.
Availability: Items available for loan: UVAS Library [Call number: 1116,T] (1).
2.
Studies Of The Carriers Of Pasteurella Multocida
by Syed Shabir Ahmad Shah | Prof. Muhammad yousaf Vaid | Mr. Muhammad | Mr. Muhammad Akram Muneer | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1979Dissertation note: Pasteurellosis is an infectious disease of Livestock having world wide occurrence. To find out the incidence of healthy carriers of Pasteurella multocida in cattle buffaloesa, research project was undertaken at College of Veterinary Sciences, Lahore.
For this purpose 330 (nasopharyngeal) swabs of clinic shy normal cattle and buffaloes were collected from various sources from Lahore.
Inoculations swabs were made on various media like blood agar, tryptore agar, tryptore broth, mutrient agar, nutrient broth, etc. The culture media wee incubated both aerobically and at a temperature of 37oC.
The biochemical characteristics of Pasteurella multocida isolated were studied, smears from growth, were prepared, stained with Gram's method and examined.
Availability: Items available for loan: UVAS Library [Call number: 1136,T] (1).
3.
Epizootiology Of Newcastle Disease In Free Flying Birds Of Pakistan
by Muhammad Arshad | Dr. Muhammad Ajmal | Mr. Mubbasher | Mr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1984Dissertation note: Epizoàtiology of Newcastle disease was studied in 105 birds each of three species of free flying birds i.e. pigeon, starling and sparrow. Incidence of Newcastle disease was measured on the basis of Haemagglutination Inhibiting antibodies against Newcastle disease virus present in the serum of each bird. It was observed that 42.86% of pigeon, 25.71% of sparrows and 21.90% of starlings were positive for Newcastle disease. An attempt was also made to isolate the virus from faecal material and pooled organs (lung,liver and spleen) of each bird in developing chick embryo. Three strains of Newcastle disease virus, 2 from pizeon and one from starling were isolated. One pigeon having virus in its organs was also found excreting Newcastle disease virus in its faeces. No virus could be isolated from sparrows. The pathogenicity of isolates was studied in day-old chicks by intracerebral inoculation and it was observed that all of the three isolates were moderately pathogenic Though non significant, the percentage of positive cases on the basis of Haemagglutination Inhibition test in male birds was found to be higher than female birds of the same species
From the tudy it was evident that pigeon could play an important role in the transmission of Newcastle disease. The other two species were also susceptible to this virus and could be source of transmission. It recommended that these as well as other species of the ree flying birds in a large number from much wider areas may be studied. It is further recommended that birds found dead in the field and pet bird markets may be investigated for virus isolation.
Availability: Items available for loan: UVAS Library [Call number: 1143,T] (1).
4.
Production, Purification And Evaluation Of Anti Tetanus Serum
by Mian Muhammad Khubaib Sattar | Prof. Dr. Tahir Yaqub | Dr | Mr. Muhammad Zubair Shabbir.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: To produce anti-tetanus serum, ten female sheep of about 3 years of age are immunized with commercially available tetanus toxoid vaccine (Imatet™, Amson vaccines and Pharma) for eighteen weeks to these experimental animals with two weeks interval. Serum samples of all the sheep were also collected fortnightly and stored at -20 ºC.
ELISA was performed to determine the antibody (IgG) titer of all the test samples. Out of 90 samples, 20 samples showed tetanus antibody (IgG) titer of 100.8 I.U. or more, while 8 samples presented tetanus antibody (IgG) titer of 160.9 IU or more. Out of these 8 samples, 3 samples had tetanus antibody (IgG) titer of 190.9 I.U. or more. Maximum tetanus antibody (IgG) titer was 195.4 I.U. at day 120.
Three samples exhibiting maximum antibody titer (190.9 I.U., 195 I.U. and 195.4 I.U.) were mixed in equal quantities for purification of Immunoglobulins (IgG). A volume of 15 mL of aggregated serum samples was mixed with 15 ml of saturated ammonium sulfate having final concentration of 45 % in the mixture which is continuously stirred at room temperature for 1 hour. Mixture was centrifuged at 10000 rpm for 30 minutes in refrigerated centrifuge machine and dialyzed against 10 changes of PBS at 4 ºC. Desalting is checked with BaCl2 solution.
The purified tetanus immunoglobulins (IgG) were treated with papain to produce Fab Fragments. Then the protein content of the purified tetanus immunoglobulins and Fab fragments was estimated with Bradford protein Assay. BSA standard curve was used to produce a regression equation [Y (OD Value) = 0.218 + 0.033 X (Protein Concentration)] which was used for calculation of the protein contents of the samples.
The purified tetanus immunoglobulins and Fab fragments were tested for purity with SDS-PAGE analysis. Then in vivo toxin neutralization test was performed in mice to check the tetanus toxin neutralization ability of the sera produced.
Availability: Items available for loan: UVAS Library [Call number: 1420,T] (1).
5.
Evaluation Of Probiotic Potential Of Locally Characterized Lactobacillus Spp. In Broiler
by Saima asghar | Dr.Muhammad Nawaz | Mr. Muhammad Asad ali | Prof. Dr.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1931,T] (1).
6.
Isolation, Molecular Identification And Antibiotic Resistance Pattern Of Salmonella Enterica From Fancy Birds
by Aqeela Kousar (2010-VA-303) | Mr. Muhammad Asad Ali | Prof. Dr. Aftab Ahmed Anjum | Prof. Dr. Mansur-ud-Din Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Salmonellosis is a disease with serious health issues related to food borne illness and
most of world’s population is suffering from it. Early diagnosis in case is very important for
treatment of disease. Salmonellosis may hidden as a carrier state, acts as zoonotic components
for transmission of disease. Therefore the test with more diagnostic value needs to be developed
like Polymerase chain reaction after culturing and microbiological examination.Salmonella
enterica infections continue to pose a significant risk for poultry industry and fancy birds.
Salmonella infections have been controlled by antibiotics but in recent times antibiotic resistance
in microorganisms especially in Salmonella is a global health issue. Antibiotic resistant
Salmonella has further compounded the problem. Poultry isolate of Salmonella enterica (n=150)
were procured from Jallo park, Safari park and household pets which are taken to Pet Centre
University of Veterinary and Animal Sciences Lahore then brought to Department of
microbiology UVAS Lahore and identified by biochemical testing, morphology, staining
characters and genus specific PCR. Antibiotic Susceptibility was checked by disc diffusion
method against amoxicillin (30μg), ampicillin (10μg), cefixime (5μg), , ceftazidime (30μg),
ceftriaxone (30μg), ciprofloxacin (5μg), gentamicin (10μg), nalidixic acid and tetracycline
(30μg) and resistant pattern was 100 % in ampicillin and tetracycline and 41.18% and 58.82% %
in gentamicin and ciprofloxacin respectively while antibiotic show 0% resistance. Fancy birds
are carriers of drug resistant Salmonellae.
A total of 150 samples collected from Zoo Lahore, safari park and household pet fancy
birds each of n=50. Samples will enriched by non-selective and selective media, After isolation
on selective media macroscopic, biochemical analysis and microscopic examination done. DNA
Summary
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extracted from culture isolated from cloacal swabs and polymerase chain reaction performed
using primers. Amplication will be observed using Agarose gel electrophoresis.
Research highlighted the prevalence of Salmonella in fancy birds and its possibility of
transmission to human beings. Research also provided data on antibiotic resistance in
Salmonellae from fancy birds and its possible role in ever increasing problem of antibiotic
resistance. Availability: Items available for loan: UVAS Library [Call number: 2615-T] (1).
